The actor has been battling colon cancer since 2016, and died at home with his family and wife by his side, according to a statement posted on his Twitter account.
He was 43, his publicist, Nicki Fioravante said in a statement.
"A true fighter, Chadwick persevered through it all, and brought you so many of the films you have come to love so much," the statement said.
"From Marshall to Da 5 Bloods, August Wilson's Ma Rainey's Black Bottom and several more, all were filmed during and between countless surgeries and chemotherapy."
The statement said the role of King T'Challa was the "honor of (Boseman's) career."
Boseman he was born in South Carolina, according to his IMDb biography. He went on to graduate from Howard University in Washington, D.C., where he returned in 2018 to give the class graduation speech -- which concluded with his iconic "Wakanda Forever" salute.
During his speech, the actor told the graduates he was fired from an unnamed production during his early acting days after he questioned what he felt was its stereotypical portrayal of black characters.
"The struggles along the way are only meant to shape you for your purpose," he had said.
The actor starred in other films, including playing James Brown in "Get On Up" and Jackie Robinson in "42."